Biosensor based on Gβγ-interacting proteins to monitor G-protein activation
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United States of America Patent
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Dec 29, 2020
Grant Date -
Aug 17, 2017
app pub date -
Oct 14, 2015
filing date -
Oct 14, 2014
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Abstract
Resonance energy transfer (RET)- or protein-fragment complement assay (PCA)-based biosensors useful for assessing the activity of G-proteins are described. These biosensors are based on the competition between the Gα subunit and a Gβγ interacting protein (βγ IP) for the binding to the Gβγ dimer. These biosensors comprises (1) a βγ IP and (2) a Gβ or Gγ protein; a GPCR; or a plasma membrane targeting domain, fused to suitable RET or PCA tags. Methods using such biosensors for different applications, including the identification of agents that modulates G-protein activity or for the characterization of GPCR signaling/regulation, such as G-protein preferences and activation profiles of GPCRs, are also described.
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