Substituted imidazo[1,2-a]pyrazines as luciferase substrates and preparation method thereof
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United States of America Patent
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Mar 20, 2018
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Feb 14, 2017
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Feb 15, 2016
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Abstract
Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogs, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods for making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
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