Methods for generating engineered enzymes
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United States of America Patent
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Jan 26, 2021
Grant Date -
Aug 17, 2017
app pub date -
Oct 14, 2015
filing date -
Oct 14, 2014
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Abstract
Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., “cleaveOme”) inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.
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