Production for recombinant human DNase I
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United States of America Patent
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Dec 8, 2020
Grant Date -
Aug 17, 2017
app pub date -
Apr 28, 2017
filing date -
Oct 31, 2014
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Abstract
Disclosed is a method for production of recombinant human DNase I which is of such high purity as may be directly used as a medical drug. The method includes the steps of; culturing recombinant DNase I-producing mammalian cells, subjecting a culture supernatant to an anion-exchange column chromatography, subjecting to a column chromatography employing as solid phase a material having affinity for phosphate group, subjecting to a cation-exchange column chromatography, and subjecting to a dye affinity column chromatography.
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