DEVICE METHOD OF MAKING ARTEPILLIN C IN PROPOLIS FOR ANTI-CANCER

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United States of America Patent

APP PUB NO 20170226042A1
SERIAL NO

15134477

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Abstract

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A method of making artepillin C in propolis includes the following steps: A. Mix propolis with ethanol to obtain a propolis-ethanol extract; B. Provide supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax and artepillin C, and then remove the wax at a bottom of the chromatographic column and collect the artepillin C containing isolate at a top of the chromatographic column; and C. Test the artepillin C by a cytotoxicity test, a cell morphology analysis, a cell cycle and apoptosis test, and a cell motility metastasis test to find an anti-cancer effect of the artepillin C containing isolate.

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Patent Owner(s)

Patent OwnerAddress
ULTRA-MICRORIGIN BIOMEDICAL TECHNOLOGY CO LTD3F NO 22 SEC 2 SHENGYI RD ZHUBEI CITY HSINCHU COUNTY 302 TAIWAN (R O C ) ZHUBEI CITY 302

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chi, Cheng-Yu Zhubei, TW 3 0
Chuang, Ming-Hsi Zhubei, TW 38 3
Chung, Hsin-Yu Zhubei, TW 3 0
Kuo, Hui-Chen Zhubei, TW 7 15
Liu, Chu-Ting Zhubei, TW 9 1
Peng, Chiu-Ying Zhubei, TW 7 0

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