METHOD FOR PREPARING REBAUDIOSIDE M BY USING ENZYME METHOD

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United States of America Patent

SERIAL NO

15114250

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Abstract

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Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.

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Patent Owner(s)

Patent OwnerAddress
PEPSICO INC700 ANDERSON HILL ROAD PURCHASE NY 10577

International Classification(s)

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  • 2014 Application Filing Year
  • C12P Class
  • 1590 Applications Filed
  • 1297 Patents Issued To-Date
  • 81.58 % Issued To-Date

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
HOU, Maoqi Jiangsu, CN 1 10
LEE, Thomas Scarsdale, US 126 1535
LI, Guoqing Jiangsu, CN 298 2993
LIANG, Xiaoliang Jiangsu, CN 12 43
TAO, Andrew San Diego, US 43 261
TAO, Junhua Jiangsu, CN 15 85
YEP, Gregory Purchase, US 9 110

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  • 10 Citation Count
  • C12P Class
  • 51.56 % this patent is cited more than
  • 8 Age
Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges13071049124399321101 - 1011 - 2021 - 3031 - 4041 - 5071 - 8081 - 90100 +010020030040050060070080090010001100120013001400

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