METHOD FOR PREPARING REBAUDIOSIDE M BY USING ENZYME METHOD
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United States of America Patent
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app pub date -
Jan 28, 2014
filing date -
Jan 28, 2014
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Abstract
Provided is a method for preparing Rebaudioside M by using an enzyme method. In the method, Rebaudioside A or Rebaudioside D is used as a substrate, and in the presence of sucrose and UDP, Rebaudioside M is generated by means of reaction of the substrate under the catalysis of a mixture of UDP-glucosyl transferase and sucrose synthetase, or recombinant cells containing the UDP-glucosyl transferase and sucrose synthetase. The reaction is carried out in an aqueous-phase system at a temperature of 20 to 60° C. and pH 5.0 to 9.0. The reaction system further contains dimethyl sulfoxide at a concentration of 3% to 5% according to the ratio by volume for facilitating solubilization of the substrate.
First Claim
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Family

- 15 United States
- 10 France
- 8 Japan
- 7 China
- 5 Korea
- 2 Other
Patent Owner(s)
Patent Owner | Address | |
---|---|---|
PEPSICO INC | 700 ANDERSON HILL ROAD PURCHASE NY 10577 |
International Classification(s)

- 2014 Application Filing Year
- C12P Class
- 1590 Applications Filed
- 1297 Patents Issued To-Date
- 81.58 % Issued To-Date
Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
HOU, Maoqi | Jiangsu, CN | 1 | 10 |
# of filed Patents : 1 Total Citations : 10 | |||
LEE, Thomas | Scarsdale, US | 126 | 1535 |
# of filed Patents : 126 Total Citations : 1535 | |||
LI, Guoqing | Jiangsu, CN | 298 | 2993 |
# of filed Patents : 298 Total Citations : 2993 | |||
LIANG, Xiaoliang | Jiangsu, CN | 12 | 43 |
# of filed Patents : 12 Total Citations : 43 | |||
TAO, Andrew | San Diego, US | 43 | 261 |
# of filed Patents : 43 Total Citations : 261 | |||
TAO, Junhua | Jiangsu, CN | 15 | 85 |
# of filed Patents : 15 Total Citations : 85 | |||
YEP, Gregory | Purchase, US | 9 | 110 |
# of filed Patents : 9 Total Citations : 110 |
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Patent Citation Ranking
- 10 Citation Count
- C12P Class
- 51.56 % this patent is cited more than
- 8 Age
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