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Abstract

A recombinant polypeptide is described which comprises at least one PUF RNA-binding domain capable of specifically binding to a cytosine RNA base. The PUF RNA-binding domain of the polypeptide comprises at least one RNA base-binding motif of the general formula X₁X₂X₃X₄X₅X₆X₇X₈X₉X₁₀X₁₁ wherein X₁ is selected from the group including glutamine (Q), valine (V), methionine (M), proline (P), glutamic acid (E), and lysine (K); X₂ is selected from the group including histidine (H), phenylalanine (F), tyrosine (Y), and asparagine (N); X₃ is selected from the group including glycine (G) and alanine (A); X₄ is selected from the group including glycine (G), alanine (A), serine (S), threonine (T) and cysteine (C); X₅ is selected from the group including arginine (R), tyrosine (Y), histidine (H), and asparagine (N); X₆ is selected from the group including phenylalanine (F), leucine (L), and valine (V); X₇ is selected from the group including isoleucine (I), leucine (L), and valine (V); X₈ is arginine (R); X₉ is selected from the group including leucine (L), lysine (K), arginine (R), glutamine (Q), and histidine (H); X₁₀ is selected from the group including lysine (K), phenylalanine (F), alanine (A), cysteine (C), isoleucine (I), valine (V), leucine (L), and methionine (M); and X₁₁ is selected from the group including leucine (L), phenylalanine (F), isoleucine (I), and valine (V); and wherein the RNA base-binding motif is operably capable of specifically binding to a cytosine RNA base.

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First Claim