METHOD AND ENZYME SOLUTION FOR FLOW-CYTOMETRIC DETECTION OF LIGHT CHAIN RESTRICTION
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May 28, 2015
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May 28, 2014
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The invention relates to an enzyme solution, which is used preferably for pre-treating mammalian cells for flow-cytometric detection of light chain restriction of the cells, the enzyme solution comprising at least two proteases, which are proteolytic and also collagenolytic, in a buffer. Preferably, the enzyme solution comprises trypsin, collagenase 4, dispase and astacin. The invention also relates to uses of the enzyme solution and methods for flow-cytometric detection of light chain restriction of cells.
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