Methods for adding adapters to nucleic acids and compositions for practicing the same
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United States of America Patent
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Sep 22, 2020
Grant Date -
Jul 13, 2017
app pub date -
Mar 24, 2017
filing date -
Oct 17, 2013
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Abstract
Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3′ hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
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