Analysis method of molecular interactions on protein nanoparticles using flow cytometry
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United States of America Patent
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Feb 2, 2021
Grant Date -
Jul 6, 2017
app pub date -
Aug 4, 2016
filing date -
Feb 7, 2014
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Abstract
The present invention relates to a method for analyzing the interaction between a binding protein and a target material, including measuring the interaction between the binding protein and a target material using an interaction trapper (IT) cell. The IT cell has the binding protein displayed on the surface of intracellular inclusion bodies, i.e., insoluble aggregates, by expressing a fusion protein which forms active protein particles containing the binding protein. The method includes increasing cell permeability without affecting the activity of the binding protein displayed on the cells and genetic information.
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