METHODS OF DEVELOPING PLANT SOMATIC EMBRYOS
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United States of America Patent
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Jul 6, 2017
app pub date -
Nov 9, 2016
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Dec 31, 2015
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Methods of developing plant somatic embryos include incubating the plant somatic embryos at a first temperature for a first period of time followed by storing the plant somatic embryos at a second temperature for a second period of time. The first temperature can be in the range of about 23° C. to 30° C. and leads to rapid plant somatic embryo growth. The second temperature can be in the range of about 8° C. to 15° C. and slows plant somatic embryo development. Plant somatic embryos developed in this manner and implanted in artificial seeds show germination rates similar to zygotic embryos implanted in artificial seeds.
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