Sandwich assays in droplets
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United States of America Patent
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Sep 1, 2020
Grant Date -
Jun 22, 2017
app pub date -
Jan 25, 2017
filing date -
Sep 30, 2010
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Abstract
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
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