Coated cell culture apparatus and methods of use
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United States of America Patent
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Aug 4, 2020
Grant Date -
Jun 15, 2017
app pub date -
Dec 15, 2016
filing date -
Dec 15, 2015
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Abstract
Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.
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