METHOD FOR ASSAYING CREATINE KINASE MB ISOZYME AND KIT TO BE USED THEREIN
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United States of America Patent
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Apr 13, 2015
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Apr 30, 2014
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The invention provides a method for measuring CK-MB in a sample by reacting the sample with a first antibody against CK-MB, a second antibody against CK-MB which recognizes an epitope different from that recognized by the first antibody, and an anti-CK-B antibody which recognizes a region of the 1st to 100th amino acid from N-terminus of the amino acid sequence of the subunit B of creatine kinase (step 1); measuring an optical change generated by the reaction (step 2); and determining a quantity of CK-MB in the sample on the basis of the result obtained in the step 2 (step 3). The invention also provides a kit to be used in the method, and a method for avoiding influence of creatine kinase BB isozyme in measuring CK-MB, comprising treating a sample with the anti-CK-B antibody.
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