Mouse cell line authentication
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United States of America Patent
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Aug 18, 2020
Grant Date -
Apr 13, 2017
app pub date -
Dec 19, 2016
filing date -
Jul 3, 2013
priority date (Note) -
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Abstract
A method, kit and primers for determining alleles present in a DNA sample that is sourced from either a mouse or a cell line derived from a mouse. A set of STR marker loci are selected that can be amplified together in a multiplex amplification reaction. The set of STR marker loci are selected from the group consisting of: 18-3, 4-2, 6-7, 15-3, 6-4, 12-1, 5-5, X-1, Mo11-1, Mo19-2, Mo1-2, Mo7-1, Mo1-1, Mo3-2, Mo8-1, Mo2-1, Mo11-2, Mo17-2, Mo13-1, D8S1106 and D4S2408. A set of oligonucleotide primer pairs are provided. Each oligonucleotide primer pair flanks a single locus in the set of STR marker loci, which are co-amplified in a multiplex amplification reaction, with the product(s) comprising a mixture of amplified alleles from each of the co-amplified loci in the set of STR marker loci. The products are evaluated to determine the alleles present at each of the loci analyzed.
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