PHYSICAL SELF-ORGANIZING HYDROGEL SYSTEM FOR BIOTECHNOLOGICAL APPLICATIONS
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United States of America Patent
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Mar 27, 2015
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Apr 1, 2014
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A physical self-organizing hydrogel system for biotechnological applications composed of a non-covalent network on the basis of a protein-ligand interaction includes a tetrameric protein and biotin or a derivative thereof as a ligand, wherein biotin or derivative is covalently conjugated to an end of a polymer chain or a linear or multi-arm synthetic polymer of a single-or double-strand oligonucleotide and wherein the solid content in relation to the entire hydrogel is at least 3% and the conjugates are crosslinked by the tetrameric protein. A mixture ratio is a molar equivalent ratio between the protein and the number of terminal biotinylated ends of the polymer chains in the biotin-polymer conjugate of 1:2 to 1:8. The hydrogel formation occurs with controlled kinetics on the basis of protein-ligand interaction.
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