Protein L based bioassay method for determining presence of soluble antibodies in a sample and kit therefor
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United States of America Patent
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Jun 2, 2020
Grant Date -
Mar 9, 2017
app pub date -
Feb 27, 2015
filing date -
Feb 27, 2014
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Abstract
This invention relates to bioassay method wherein the presence of a specific soluble antibody in a sample of a bodily fluid, plasma or serum, of an animal, including human, is qualitatively and/or quantitatively determined. The method employs, a first group labelled with an energy donor and a second group labelled with an energy acceptor; the first group, or second group, comprising an antigen of the soluble antibody and the second group, or first group, respectively, comprising a Fab binding moiety capable of binding to a Fab region of antibodies of said animal. The donor and the acceptor form an energy transfer pair capable of energy transfer. The invention further comprises a kit for the bioassay method.
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