ISOLATION OF NUCLEIC ACID

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United States of America Patent

SERIAL NO

15207091

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Abstract

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A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.

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Patent Owner(s)

Patent OwnerAddress
CAMBRIDGE ENTERPRISE LIMITEDTHE OLD SCHOOLS TRINITY LANE CAMBRIDGE CAMBRIDGESHIRE CB2 1TN

International Classification(s)

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  • 2016 Application Filing Year
  • C12N Class
  • 7780 Applications Filed
  • 4946 Patents Issued To-Date
  • 63.58 % Issued To-Date
Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances20162017201820192020202120222023202420250255075100

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
CHUA, Yii Leng Cambridge, GB 5 8
RITCHIE, Allyson Victoria Cambridge, GB 4 6

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Patent Citation Ranking

  • 3 Citation Count
  • C12N Class
  • 53.39 % this patent is cited more than
  • 8 Age
Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges2925224021854181131251101 - 1011 - 2021 - 3031 - 4041 - 5051 - 6061 - 7071 - 8081 - 9091 - 100100 +0200400600800100012001400160018002000220024002600280030003200

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