TLR-4 BASED ELECTROCHEMICAL BIOSENSOR
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United States of America Patent
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Feb 2, 2017
app pub date -
Jun 25, 2016
filing date -
Jun 26, 2015
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Abstract
The present invention relates to an apparatus comprising TLR-4 based electrochemical biosensor and a method for using the same to detect the presence of gram-negative bacteria or lipopolysaccharide in a sample. In one particular embodiment, the apparatus comprises an electric conducting solid substrate surface having a monolayer of a mixture of linkers each of which has a first functional group that is attached to the surface of said electric conducting solid substrate. The mixture of linkers comprises tethering-linkers and spacer-linkers such that the amount of spacer-linkers is equal to or greater than that of the tethering-linkers. The tethering linker also comprises a second functional group that is used to attach a toll-like receptor 4 (TLR-4).
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