AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS
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United States of America Patent
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Issued Date -
Feb 2, 2017
app pub date -
Oct 17, 2016
filing date -
Feb 23, 2010
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Abstract
Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells.
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