NUCLEIC ACID AMPLIFICATION METHOD
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United States of America Patent
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Jan 7, 2015
filing date -
Jan 23, 2014
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Abstract
The invention relates to a method for the simultaneous detection of at least four nucleic acids labeled with a covalently attached dye in a sample comprising the step of detecting the fluorescence emission of said fluorescent dyes upon excitation, wherein the at least four dyes attached to said nucleic acids are selected from the groups of i) 6-FAM or 5-FAM or a blend thereof covalently attached to a first nucleic acid, ii) DY-530, HEX or ATTO 532 or a blend thereof covalently attached to a second nucleic acid, iii) ATT0550 or DY-555 or a blend thereof covalently attached to a third nucleic acid, iv) ATT0565, DY510-XL or ROX or a blend thereof covalently attached to a fourth nucleic acid, v) DY632 or DY520-XL or a blend thereof covalently attached to a fifth nucleic acid and, vi) Chromeo 494 attached to a sixth nucleic acid, wherein Chromeo 494 must be one of the four selected.
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