IMMUNOCHROMATOGRAPHIC DETECTION METHOD
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United States of America Patent
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Issued Date -
Dec 29, 2016
app pub date -
Dec 1, 2014
filing date -
Nov 29, 2013
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Abstract
The present invention provides a detection method using an immunochromatographic test strip that suppresses the occurrence of a so-called white bleaching phenomenon and that is accurate and excellent in sensitivity. The present invention provides an immunochromatographic detection method that uses an immunochromatographic test strip including (1) and (2) below and that comprises the steps of (A) and (B) below: (1) a conjugate pad having a sample-supply portion for supplying a sample possibly containing an analyte, and a conjugate portion on the downstream side of the sample-supply portion, the conjugate portion containing a conjugate in which an antibody to the analyte is immobilized on a label, (2) an insoluble membrane support having at least one detecting section on which an antibody to the analyte is immobilized, (A) a step of supplying methanol and the sample possibly containing the analyte to the sample-supply portion, and (B) a step of detecting the analyte as an immune reaction product on the insoluble membrane support.
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