REDUCING OVERFRAGMENTATION IN ULTRAVIOLET PHOTODISSOCIATION
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United States of America Patent
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Dec 8, 2016
app pub date -
Jun 2, 2016
filing date -
Jun 3, 2015
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Abstract
A method and apparatus are disclosed for dissociation of precursor ions, such as polypeptides, by ultraviolet photodissociation (UVPD) for mass spectrometry analysis. Precursor ions are confined within an ion trap and irradiated with ultraviolet (UV) light, which may take the form of pulses emitted by a laser. The precursor ions absorb the UV light and dissociate into product ions. To avoid the condition of overfragmentation arising from further dissociation of the product ions, an excitation field is established within the ion trap such that the product ions, but not the precursor ions, are kinetically excited to trajectories that extend outside of the irradiated region.
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