Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
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United States of America Patent
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Nov 17, 2020
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Dec 8, 2016
app pub date -
Jul 22, 2016
filing date -
Mar 7, 2003
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Abstract
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
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