Caution chelator hot start
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United States of America Patent
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Apr 28, 2020
Grant Date -
Nov 24, 2016
app pub date -
Jan 15, 2015
filing date -
Jan 31, 2014
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Abstract
The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.
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