METHOD FOR REFOLDING RECOMBINANTLY PRODUCED POLYPEPTIDES
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United States of America Patent
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Nov 24, 2016
app pub date -
Dec 22, 2014
filing date -
Dec 22, 2014
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Abstract
Disclosed is a method for refolding recombinantly produced polypeptides comprising the steps of (a)providing inclusion bodies comprising recombinantly produced polypeptides comprising the recombinantly produced polypeptides, (b)dissolving the inclusion bodies under chaotropic conditions in so as to obtain denatured recombinantly produced polypeptides, and (c)refolding the denatured recombinantly produced polypeptides inclusion bodies by addition of a refolding buffer,so as to obtain refolded re-combinantly produced polypeptides, and (d) further processing the refolded recombinantly produced polypeptides by at least one processing step, wherein at least steps (c) and (d) are performed in a tubular reactor in a continuous manner.
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