METHOD FOR PURIFYING OXIDIZED FORM OF BETA-NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE
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United States of America Patent
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Nov 24, 2016
app pub date -
Dec 4, 2015
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May 19, 2015
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Abstract
The present invention discloses an oxidized form of β-nicotinamide adenine dinucleotide phosphate and a method for purifying the same. The method comprises specifically the steps of: a. sequentially microfiltrating and nanofiltrating a pretreated coenzyme II solution with membrane concentration devices, to obtain a concentrated crude product solution; b. adjusting the obtained crude product solution to pH 2-4, loading it onto a preparative reverse phase high performance liquid chromatographic column, and purifying by gradient elution, to obtain a purified sample solution; and c. nanofiltrating the purified sample solution with a membrane concentration device, and freeze drying it in a vacuum freeze drier, to obtain a purified coenzyme II. The coenzyme II prepared in the present invention has a high purity, a high yield, and thus a promising prospect in the market.
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