USE OF TRICARBOXYLIC ACID (TCA) INTERMEDIATES TO CONTROL AMMONIA GENERATION IN CELL CULTURE
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United States of America Patent
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Oct 27, 2016
app pub date -
Mar 13, 2014
filing date -
Mar 15, 2013
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Abstract
The present invention is directed to a method of reducing the ammonium ion concentration in a mammalian cell culture, a method of maintaining or increasing cell productivity in a mammalian cell culture, a method of maintaining or increasing cell growth in a mammalian cell culture, and a method of reducing the influence of ammonium ion accumulation on antibody glycosylation patterns in a cell culture, comprising steps of growing the cells in a cell culture medium, and contacting or administering an effective amount of a TCA intermediate composition to the cell culture medium.
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