Compositions and methods for detecting rare sequence variants
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United States of America Patent
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Sep 8, 2020
Grant Date -
Oct 20, 2016
app pub date -
Dec 11, 2014
filing date -
Dec 11, 2013
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Abstract
In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods.
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