Method and kit for detection of microorganism
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United States of America Patent
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Jun 25, 2019
Grant Date -
Oct 13, 2016
app pub date -
Jun 21, 2016
filing date -
Jul 24, 2009
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Abstract
A kit for detecting live cells of a microorganism in a test sample by distinguishing the live cells from dead cells or injured cells by a nucleic acid amplification method includes the following components:
- 1) an agent capable of covalently binding to DNA or RNA of the microorganism by irradiation with light having a wavelength of 350 nm to 700 nm;2) an agent for suppressing a nucleic acid amplification inhibitory substance; and3) a primer or primers for amplifying a target region of the DNA or RNA of the microorganism to be detected by a nucleic acid amplification method. The agent for suppressing a nucleic acid amplification inhibitory substance is one or more selected from albumin, dextran, T4 gene 32 protein, acetamide, betaine, dimethyl sulfoxide, formamide, glycerol, polyethylene glycol, soybean trypsin inhibitor, α2-macroglobulin, tetramethylammonium chloride, lysozyme, phosphorylase and lactate dehydrogenase.
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