METHODS OF SELECTING AKT AGONISTS OR ANTAGONISTS
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United States of America Patent
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Issued Date -
Oct 6, 2016
app pub date -
Mar 30, 2016
filing date -
Mar 31, 2015
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Abstract
Disclosed herein are methods of identifying a test compound as agonists or antagonists of Akt activity. The methods involve contacting the test compound with a cell that expresses a biosensor comprising a FOXO1 or HDHB polypeptide and a fluorescent protein and locating the biosensor within the cell. Locating the biosensor in the nucleus relative to the cytoplasm is an indication that the test compound has an effect upon Akt activity.
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