OLIGONUCLEOTIDES COMPRISING A SECONDARY STRUCTURE AND USES THEREOF
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United States of America Patent
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Issued Date -
Oct 6, 2016
app pub date -
Aug 27, 2014
filing date -
Aug 27, 2014
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Abstract
There is provided a single stranded oligonucleotide having a 5′ end and a 3′ end, said oligonucleotide comprising a first and second section, the first section being positioned 5′ of the second section; and wherein (i) the first section is labelled with at least two detectable labels and is capable of hybridising to a target polynucleotide; and (ii) the second section is not capable of hybridising to a target polynucleotide; said second section comprising a stem-loop structure comprising a first portion, a second portion and a third portion and wherein the second portion is located between the first and third portions, and the first and third portions are complementary to each other. There is also provided a method of detecting the presence of a target polynucleotide and/or sequence variations within a target polynucleotide using such an oligonucleotide.
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