Bead Manipulation Techniques

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United States of America Patent

APP PUB NO 20160271623A1
SERIAL NO

15165883

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

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Patent Owner(s)

Patent OwnerAddress
ADVANCED LIQUID LOGIC INCRESEARCH TRIANGLE PARK NC

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Pamula, Vamsee K Cary, US 132 12386
Sista, Ramakrishna Cary, US 45 1544
Sudarsan, Arjun Carlsbad, US 44 1661
Thwar, Prasanna Los Altos, US 29 1463

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