METHODS FOR REDUCING CONTAMINATION DURING ENZYMATIC HYDROLYSIS OF BIOMASS-DERIVED CELLULOSE
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United States of America Patent
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Sep 8, 2016
app pub date -
Mar 6, 2016
filing date -
Mar 6, 2015
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Abstract
Some variations provide a method of enzymatically converting biomass-derived cellulose to glucose, comprising exposing the biomass-derived cellulose to (i) cellulase enzymes, to hydrolyze the cellulose to glucose; and (ii) an external sulfur-containing compound, to deter bacterial and/or yeast contamination during cellulose hydrolysis. In some embodiments, the sulfur-containing compound includes sulfur dioxide or lignosulfonates. When the sulfur-containing compound includes lignosulfonates, the lignosulfonates may also function as an enzyme surfactant to assist hydrolysis, in addition to deterring bacterial and/or yeast growth/contamination. This method may be applied to cellulose-rich solids obtained from the AVAP® fractionation process, the Green Power+® pretreatment process, or any other source of cellulose-rich solids.
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