Method for detecting time-resolved fluorescence based on principle of phase balanced frequency multiplication modulation
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Jan 16, 2018
Issued Date -
N/A
app pub date -
Jul 23, 2014
filing date -
Jul 23, 2014
priority date (Note) -
In Force
status (Latency Note)
Importance
|
US Family Size
|
Non-US Coverage
|
|
Patent Longevity
|
Forward Citations
|
Abstract
The present invention relates to a method for detecting time-resolved fluorescence based on a principle of phase balanced frequency multiplication modulation. A stimulating light source modulated by using a baseband signal acts on a to-be-measured target to trigger fluorescence, so that the fluorescence intensifies and decays periodically; then, a frequency-doubled square signal is used to control a sampling period and divide an ascending period of the fluorescence into two and a decay period of the fluorescence into two; after independent sampling is performed separately, sampling differences of the two parts are separately calculated and then added to obtain an intensity representative value of a fluorescence signal and to obtain a concentration value of the to-be-measured target. The method in the present invention can not only likewise cancel fluorescence interference of a substrate in a sample, but also can cancel ambient bias light, power-frequency interference of a spatial electromagnetic wave or other signals, and therefore improves signal intensity in fluorescence measurement on the detection sample, has an advantage that cannot be accomplished in a conventional time-resolved fluorescence method, and can be applied in fluorescence intensity detection of a target in fields such as biology, chemistry, and medicine.
First Claim
Other Claims data not available
Family
| Country | kind | publication No. | Filing Date |
|---|---|---|---|
| WO | A1 | WO2016011622 | Jul 23, 2014 |
International Classification(s)
- 2014 Application Filing Year
- G01J Class
- 1566 Applications Filed
- 1387 Patents Issued To-Date
- 88.57 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 0 Citation Count
- G01J Class
- 0 % this patent is cited more than
- 7 Age
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | Jul 16, 2025 |
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Jul 16, 2029 |
