METHOD FOR STEADYING THERMAL CONVECTION FLOW FIELD IN SOLUTION DURING THERMAL CONVECTIVE POLYMERASE CHAIN REACTION
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May 2, 2016
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Mar 9, 2012
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Abstract
A method for steadying a thermal convection flow field in a PCR reaction solution during a thermal convective polymerase chain reaction (PCR) includes steps as follows. A PCR tube is provided. A PCR reaction solution is filled in the PCR tube. A bottom of the PCR tube is heated so that a thermal convection is induced. And the PCR to be is tilted at a tilt angle relative to a vertical line over a ground for causing a single-loop flow in the thermal convection flow field in the PCR reaction solution, whereby a denaturation an annealing reaction and an extension reaction occur sequentially and repeatedly in different temperature regions of the PCR reaction solution.
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