CORNEAL STROMAL KERATOCYTE CULTURE
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United States of America Patent
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Aug 11, 2016
app pub date -
Sep 17, 2014
filing date -
Sep 17, 2013
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Abstract
The present invention relates to corneal stromal keratocytes (CSKs) culture. In particular the present invention relates to a method for culturing corneal stromal keratocytes ex vivo. The method utilises a dual culture medium protocol. In the presence of serum (in particular low serum concentration) in a first culture medium A, the “activated keratocytes” are expandable while specific CSK gene expression is maintained in serum-free ERI condition in a second culture medium B. The invention also relates to culture medium A and B which is supplemented with liquid amnion extract or other additional supplements. This protocol also applied to CSK from other species. Additionally, the present invention provides method for CSK cultivation to provide sufficient quantity of genuine CSKs for corneal tissue engineering without a risk of fibroblastic changes.
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