METHOD OF ISOLATING CIRCULATING TUMOR CELLS
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United States of America Patent
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Aug 4, 2016
app pub date -
Feb 3, 2016
filing date -
Feb 4, 2015
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Abstract
A method of purifying circulating tumor cells is disclosed. The method involves purifying a first buffy coat layer from a blood sample on a first density gradient. The blood sample comprises a circulating tumor cell. The first buffy coat layer is reconstituted in whole blood to yield an enriched sample. The volume of whole blood used to reconstitute the first buffy coat layer is less than 20% of the volume of the blood sample. Circulating tumor cell enrichment reagent is added to the enriched sample at a volume of less than 20% of the total volume of the enriched sample. The enriched sample is placed on a second density gradient and a second buffy coat layer is collected. The second buffy coat layer comprises the circulating tumor cell.
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