Medium for culturing the Marc-145 cell and the preparation method thereof
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United States of America Patent
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Aug 4, 2016
app pub date -
Oct 30, 2015
filing date -
Jan 30, 2015
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Abstract
The present invention relates to a medium for culturing the M-arc-145 cell, which consists of the following components: the Dulbecco Modified Eagle Medium (DMEM), the solution of gelatin digested by the enzyme and the fetal calf serum; wherein the volume ratio of said DEME, said solution of hydrolytic gelatin by the enzyme and said fetal calf serum is 1 to 2:1 to 5:2 to 5:100. The present invention furthermore provides a preparation method thereof, that is: obtaining the medium by means of taking the solution of gelatin digested by the enzyme, the DEME solution containing the amino acid and the fetal calf serum with the measuring cylinder according to the quantities in the formula, and mixing these components. The medium for culturing the M-arc-145 cell of the present invention may reduce the usage amount of the fetal calf serum during the preparation, hence decreasing the production cost, and of which the preparation method is rather simple and easy to operation.
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