METHOD FOR MASS PRODUCTION OF FACTOR VII/VIIA DERIVATIVES
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United States of America Patent
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Jul 28, 2016
app pub date -
Aug 27, 2014
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Aug 27, 2014
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Abstract
A method for mass production of human coagulation factor VII derivatives. The method employs an expression vector, containing i) a nucleotide sequence of DHFR promoter devoid of at least one CCGCCC repeat sequence from the GC-rich region thereof and a nucleotide sequence encoding a DHFR operably linked thereto, and ii) a nucleotide sequence of CMV early gene promoter and a nucleotide sequence encoding a human coagulation factor VII derivative operably linked thereto; and adds at least one selected from the group consisting of sodium butyrate, vitamin K, and a culture medium supplement.
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