METHODS FOR GENERATING 3D CELL CULTURE MODEL IN HIGH RESPIRATION ENVIRONMENTS FOR HIGH-CONTENT ANALYSES AND SCREENING
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United States of America Patent
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Issued Date -
Jul 28, 2016
app pub date -
Jan 23, 2015
filing date -
Jan 23, 2015
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A method of culturing cells to form cell aggregations and subjecting the cell aggregations to imaging analysis, comprises: seeding cells in a culture medium on a substantially flat substrate that is transparent, oxygen gas-permeable and liquid impermeable and is coated with atelo-collagen of large-mammalian origin; culturing the cells by continuously supplying air or oxygen-containing gas to the cells through said substrate to form the cell aggregations that are three-dimensional (3D), shaped as undulated as islands or mountains and discretely arranged as islands separated by substantially cell-free gaps; subjecting the cell aggregations on said substrate to the imaging analysis by using lights passing through said substrate.
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