Method for animal tissue material disinfection and sterilization and corresponding animal tissue soak solution
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United States of America Patent
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Jul 7, 2020
Grant Date -
Jul 28, 2016
app pub date -
May 29, 2014
filing date -
Aug 26, 2013
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Abstract
A method for disinfecting and sterilizing an animal tissue material and a corresponding soak solution for an animal tissue. The method includes placing the animal tissue material into an alkaline soak solution containing a metal peroxide and a detergent, and shaking; removing organic components released from microorganisms and animal tissue cells by soaking and washing in a neutral cleaning solution; washing the tissue matrix with a weak acidic cleaning solution; cryopreserving or freeze-drying the tissue matrix in a neutral solution. The soak solution for the animal tissue contains 0.01˜0.2% (w/v) of the metal peroxide and 0.05˜1.0% (w/v) of the detergent. The animal tissue pretreated by the method is advantageous to the preservation and further decellularization treatment of the tissue for manufacture of an intact scaffold material of a tissue matrix.
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