DETECTION OF BIOLOGICAL MOLECULES USING SURFACE PLASMON FIELD ENHANCED FLUORESCENCE SPECTROSCOPY (SPFS) COMBINED WITH ISOTACHOPHORESIS (ITP)
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United States of America Patent
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Issued Date -
Jul 14, 2016
app pub date -
Jan 13, 2016
filing date -
Jan 13, 2015
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Abandoned
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Abstract
A method for detecting biological molecules that combines surface plasmon field-enhanced fluorescence spectroscopy (SPFS) and Isotachophoresis (ITP). An ITP setup, including a TE reservoir, an LE reservoir, and a fluid channel connecting the two, is equipped on the SPFS sensor, such that the solution in the fluid channel passes the SPFS sensor surface between the TE reservoir and the LE reservoir. Target analytes and fluorescent labeled probes loaded into the TE reservoir are focused in a region of fluid channel upstream from the SPFS sensor region, and they are reacted. The focused sample travels downstream to reach the SPFS sensor region, and the analyte-probe complexes are captured by capture molecules immobilized on the sensor surface. After the focused sample completely passes through the SPFS sensor region, captured fluorescent molecules on the sensor surface are detected using the SPFS mechanism.
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