Salmonella and Listeria Assay Methods and Kits
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United States of America Patent
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Jul 14, 2016
app pub date -
Sep 30, 2015
filing date -
Sep 30, 2014
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Abstract
Methods and kits for detection of bacteria, especially Salmonella spp. and Listeria spp., are provided using a unique combination of selective ingredients and two-phase culture (solid-phase culture gel and liquid-phase culture/enrichment broth) allows for high sensitivity and specificity of the kits for growth of Salmonella and Listeria in the detection methods and kits. The invention, through the detection mechanism accomplished by the novel formulation of selective ingredients and the two-phase culture, allows for real-time detection of a single cell of Salmonella and Listeria within 24±2 hours of introducing a target sample to the Salmonella and Listeria detection kits. The detection methods and kits for Salmonella may optionally further comprise a confirmation step that can increase sensitivity and accuracy for this pathogenic bacteria.
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