METHOD OF PRETREATMENT OF SAMPLE FOR QUANTITATING CHOLESTEROL AND METHOD FOR QUANTITATING CHOLESTEROL IN SPECIFIC LIPOPROTEINS BY USING THE SAME

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United States of America Patent

SERIAL NO

14803179

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Abstract

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A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.

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Patent Owner(s)

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SEKISUI MEDICAL CO LTD1-3 NIHONBASHI 2-CHOME CHUO-KU TOKYO 103-0027

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
MANABE, Mitsuhisa Ryugasaki-shi, JP 19 79
NAKAMURA, Mitsuhiro Ryugasaki-shi, JP 172 2519
TANIGUCHI, Yuriko Ryugasaki-shi, JP 10 46
YAMAMOTO, Mitsuaki Ryugasaki-shi, JP 53 33

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