Cell Culturing Method, Particulate Culture Carrier, and Particle-Encompassing Cell Aggregate
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United States of America Patent
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Issued Date -
Jun 16, 2016
app pub date -
May 9, 2014
filing date -
May 23, 2013
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Abandoned
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Abstract
Provided is a culturing method capable of causing a three-dimensional aggregate in which cell function is similar to in vivo function to adhere to a culturing vessel while improving permeability of nutrients, oxygen, drugs and reagents from the culture medium and maintaining cell function. By sowing isolated cells in a culturing vessel, and after the cells have settled on the culturing surface, administering microscaffold particles that are cell-adhesive and substance-permeable onto the cells, it is possible to cause the cells to adhere to the culturing vessel while forming cell aggregates that encompass the particles. For example, cell aggregates encompassing the particles are formed by administering gelatin particles in which the size of the culture carrier to be encompassed in the cell aggregate is at least ⅕ of the cell diameter at a cell number:particle number of at least 80:1 with respect to the number of cultured cells per unit culturing area.
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