Methods for enhancing alpha-ketoglutarata production in Yarrowia lipolytica
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May 19, 2016
app pub date -
Dec 2, 2014
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Dec 2, 2014
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Abstract
The present invention provides methods for enhancing α-KG production in Yarrowia lipolytica, relates to the field of metabolic engineering. This invention successfully overexpresses the glutamate dehydrogenase in wild type strain Y. lipolytica WSH-Z06 to construct the recombinant Y. lipolytica WSH-Z06 which regulates the glutamate catabolism to synthesis α-KG. L-methionine imine is added into the fermentation medium during the process to strengthen the supply of intracellular glutamate and inhibite the intracellular glutamine synthesis from glutamate metabolism and then enhance the accumunation of α-KG. Therefor, the present invention provides an effective method for enhancing the accumunation of α-KG through regulation of intracellular amino acid metabolism.
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