METHODS FOR PHENOTYPING OF INTACT BONES BY TISSUE CLEARING AND STAINING
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United States of America Patent
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May 12, 2016
app pub date -
Nov 6, 2015
filing date -
Nov 7, 2014
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Abstract
In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone. In some embodiments, the method includes serially incubating cleared bone in refractive index matching solutions with progressively higher refractive indexes. In some embodiments, the methods teach immunolabeling and/or staining tissue containing bone and optionally visualizing the immunolabeled and/or stained tissue containing bone.
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