METHOD AND APPARATUS FOR AMPLIFYING DNA FRAGMENT BASED ON CONTROLLING PH CHANGE OF REACTION SOLUTION
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United States of America Patent
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May 12, 2016
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Jun 9, 2014
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Jun 9, 2014
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The present invention provides a method and apparatus based on a DNA fragment amplified by changing the pH value of a control reaction solution. Specifically, the present invention provides a method for nucleic acid amplification, comprising the following steps: (a) under conditions of pH 10-14 alkalinity, melting of a double-stranded nucleic acid molecule; (b) under conditions of pH 5-8 neutrality and near-neutrality, annealing of the melted nucleic acid molecule and a primer; and in the presence of a nucleic acid polymerase, causing the primer bound to the single-stranded nucleic acid molecule to extend to form an amplified double-stranded nucleic acid molecule.
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