METHODS OF MEASURING GENE EXPRESSION IN FACS-SORTED CELLS
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United States of America Patent
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May 12, 2016
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Apr 2, 2014
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Apr 2, 2014
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Improved methods of measuring gene expression in intracellularly immunostained FACS-sorted cell populations are provided. Exemplary methods involve fixing cells with formalin and permeabilizing with mild detergent in the presence of ribonucleoside vanadyl complex prior to FACS sorting, followed by RNA extraction in the presence of de-crosslinking agents. The resulting RNA is suitable for gene expression analysis. The method allows for analysis of the gene expression pattern specifically associated with any sortable cell population or subpopulation.
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